An Introduction to Microbiome Analysis workshop will be presented as part of the BIOS² training program by Professor Steven Kembel (UQAM) on May 19 and 20, 2022 from 1 pm to 4 pm Eastern Time .
This workshop will give an overview of the theory and practice of using metabarcoding approaches to study the diversity of microbial communities. The workshop will give participants an understanding of 1) the current methods for microbiome diversity quantification using metabarcoding/amplicon sequencing approaches and 2) the normalization and diversity analysis approaches that can be used to quantify the diversity of microbial communities.
This online workshop will take place from 1pm-4pm Eastern time on May 19 and 20, 2022. Each day there will be a ~1 hour lecture followed by a ~2 hour hands-on practical using R.
- May 19 : Microbiome quantification using metabarcoding/amplicon sequencing approaches
- May 20: data normalization and ecological analysis of microbiome data
The workshop assumes basic familiarity with R/RStudio; practical exercises are based on the use of R scripts to analyze sequence data and resulting community data sets.
Participants will need access to a computer with the ability to install recent versions of R/RStudio and the dada2 and picante packages.
The workshop will be taught in English with discussion and questions in English and French by Pr. Steven Kembel and PhD Student and BIOS2 Fellow Zihui Wang.
Pr. Steven Kembel is a biology professor at Université du Québec à Montréal (UQAM). He is part of the BIOS2 core team as a co-investigator. Dr. Steven Kembel, Canada Research Chair in Plant Microbiomes, is studying the biodiversity of microbes living on plants. His research uses environmental DNA sequencing to understand the processes that support the biodiversity of plant-associated microbes and the effects microbes have on plant hosts.